Hematology Analyzer (CBC) analysis instrument detection method

A Hematology Analyzer (CBC) analyzer, also known as a Hematology Analyzer (CBC) cell analyzer, hemocytometer, blood cell counter, etc., is one of the most widely used instruments in hospital clinical testing Laboratory Devices. With the rapid development of computer technology in recent years, the technology of Hematology Analyzer (CBC) analysis is also from triads to quintiles, from 2D to 3D, and we also note that the quintuples of modern Laboratory devise analyzers use many of the same techniques as today's very advanced flow cytometers, such as scattered light detection technology, sheath flow technology, laser technology, etc. This paper focuses on the detection methods and applications of the Hematology Analyzer (CBC) and analytical instruments.

Here is the content list:

l Volume, conductance, laser scattering (VCS).

l Combined detection technology of electrical impedance, radio frequency, and cytochemistry.

Volume, conductance, laser scattering (VCS).

This is the classic analysis method adopted by the Hematology Analyzer (CBC) analyzer, which integrates three physical detection techniques to perform multi-parameter analysis of cells in their natural and primitive state. This method is also known as volume, conductance, and laser scattering Hematology Analyzer (CBC) analysis. In this technique, a red blood cell lysing agent is added to the specimen to dissolve the red blood cells, and then a stabilizer is added to neutralize the effect of the red blood cell lysing agent, so that the white blood cell surface, cytoplasm, and cell volume remain stable. Then, the cells were pushed into the flow cytometry cell by the sheath flow technique and detected by the three techniques of the instrument VCS.

Combined detection technology of electrical impedance, radio frequency, and cytochemistry.

There are four different detection systems in this type of instrument. The samples are treated with special cell staining techniques and then RF and DC techniques are applied to classify and count white blood cells. It adopts the following four detection systems. The first is the eosinophil detection system, which uses electrical impedance to count. Hematology Analyzers (CBC) are partially mixed with eosinophil count hemolytic agent after the blood is divided by the blood divider. The specific hemolytic agent causes all cells except eosinophils to be lysed or atrophied. This liquid containing intact eosinophils impeded, circuit count. Then there is the basophil system, which detects the same principle as eosinophils, except that its hemolytic agent can only retain basophils in Hematology Analyzer (CBC). Next is the detection system for lymph, monocytes, and granulocytes. The system adopts the combined detection method of electrical impedance and radio frequency and uses a milder hemolytic agent, which has little effect on the nuclear and cell morphology. There are both DC and high-frequency transmitters on the inner and outer electrodes. Since the direct current cannot reach the cytoplasm and nucleoplasm, and the RF energy of the Hematology Analyzer (CBC) penetrates the cell to measure the nuclear size and the number of particles, the number, and level of these two different pulse signals comprehensively reflect the cell number, size (DC) and Nuclear and particle density (RF). Because lymphocytes, monocytes, and granulocytes are quite different in size, cytoplasmic content, nuclear morphology, and density, their proportions can be obtained by scanning. Finally, there is the naive cell detection system, which also uses electrical impedance to count. The principle is that naive cells have fewer lipids than mature cells. After adding sulfurized amino acids to the cell suspension, due to different lipid occupancy, more sulfurized amino acids are bound to immature cells than mature cells, and they are more resistant to hemolytic agents. Therefore, it can keep the shape of immature cells intact and dissolve mature cells, which can be detected by the impedance method.

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